A responsive yet persistently recurrent GBM with PNET features

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A 49-year-old male presented to his primary provider with a 6-month history of unilateral resting tremor and tingling facial sensations. Cranial MRI revealed a 6 × 3.1-cm hemorrhagic lesion in the right temporal lobe (Figure 1). The patient underwent gross total resection (GTR) revealing tissue consistent with glioblastoma multiforme (GBM). While the large lesion was in close proximity to multiple meningeal structures, neither imaging nor intraoperative assessments strongly indicated leptomeningeal disease. However, neuroblastic foci positive for synaptophysin with a Ki-67 proliferation index approaching 100% were also identified. As a result, the diagnosis of GBM with primitive neuroendocrine tumor (PNET) features was made. The patient underwent adjuvant volumetric-modulated arc radiation therapy (VMAT) at 59.4 Gy in 1.8 Gy fractions followed by a stereotactic radiosurgery (SRS) boost at 17 Gy to the 50% iso-dose line to an area of residual enhancement. Adjuvant temozolamide was also provided.

Imaging performed 1 month after completion of the patient’s initial course of treatment showed dural-based lesions in bilateral frontal lobes that were also hypermetabolic on positron emission tomography (PET) (Figure 2). Hence, approximately 8 months from initial surgical resection, the patient underwent a left-sided craniotomy and GTR of the largest lesion, which confirmed an out-of-field, contralateral recurrence of his GBM with PNET features. Another round of adjuvant radiation via VMAT at 55.8 Gy with concurrent temozolamide was initiated, targeting the resection cavity and the right-sided lesion.

The patient retained stable functional capacity with no new neurological symptoms or complaints. However, follow-up imaging showed a local recurrence in the right temporal lobe where the initial resection had been performed 12 months earlier (Figure 3A). The patient started a regimen of etoposide and vincristine, but after 1 cycle, he developed seropurulent drainage from his craniotomy site with projectile vomiting, neck stiffness and low back pain. He was diagnosed with acute meningitis requiring craniectomy with bone washing and intravenous antibiotic therapy. After recovering, he began a regimen of procarbazine, lomustine, and vincristine (PCV) with bevacizumab. Subsequently, resolution of the previously noted contrast enhancement had resolved, and all therapies were discontinued.

Nevertheless, in the follow-up 24 months after his initial craniotomy, imaging revealed another local recurrence with contrast enhancement in the temporal lobe (Figure 3B). With minimal morbidity and stable performance status, the patient wished to pursue aggressive treatment, so the recurrent area was given a third round of VMAT to 39.6 Gy with concurrent temozolamide and bevacizumab, followed by adjuvant etoposide, carboplatin and bevacizumab.

Two months following therapy, the patient complained of weakness, somnolence, weight loss and low back pain. Brain imaging remained stable, but spinal imaging demonstrated extensive leptomeningeal disease. Although a palliative course of radiation was initiated, the patient succumbed to his disease shortly thereafter, approximately 28 months following initial diagnosis and resection.


Initial diagnostic MRIs showed a heterogeneously enhancing mass in the right temporal lobe with peritumoral edema, midline shift, and uncal herniation. The T1 phase of this exam is provided in Figure 1. Functional MRI also revealed language localization of the left, and the mass to be separated from the motor strip. The patient’s initial recurrences in bilateral frontal lobes were also enhancing, yet more homogenously, and were closely approximated to the cerebral meninges. This exam, as well as a PET/CT of the brain showing the increased metabolic activity at these sites, is shown in Figure 2. After treatment for these lesions, follow-up imaging showed a local recurrence at the initial site following a second round of radiation and temozolamide therapy (Figure 3, left), followed by persistent disease after an additional 12 months of chemotherapy and a lengthy hospitalization for meningitis (Figure 3, right). And finally, the patient’s disease eventually spread to his sacral spine via leptomeningeal dissemination, which was seen on lumbosacral MRI exams as “sugar-coating” confluent lesions of the cauda equina and thickening of the surrounding meninges and dura.


Multipally recurrent glioblastoma multiforme (GBM) of the right temporal lobe with primitive neuroendocrine tumor (PNET) features and leptomeningeal dissemination.


Currently, standard treatment for GBM includes local radiation therapy and temozolamide. Although GBM has classically been considered a purely astrocytic tumor, lesions with mesenchymal and epithelial constituents are increasingly being described. GBM with PNET components are thought to represent approximately 0.5% of GBM cases, and arise from the development of PNET-like foci within pre-existing gliomas. In contrast to conventional GBM, PNETs typically have a histology similar to medulloblastoma and are often managed with combined craniospinal radiation and platinum-based chemotherapy.

The majority of GBM tumors are resistant to therapy, and mean survival is approximately 15-17 months. Although the prognosis for PNET patients is also fairly poor, the response rate to therapy is more marked, with an estimated 4-year survival rate of 38%. An established clinical feature of PNETs is a high risk for metastatic spread into the cerebrospinal fluid (CSF). Although the clinical data is sparse, recent studies have suggested that patients with GBM-PNETs are also at increased risk for CSF dissemination. In a recent multi-institutional series of 53 patients with GBM-PNETs, Perry et al reported leptomeningeal metastasis in up to 40% of patients. This frequency is in sharp contrast to the approximate 1% CSF spread rate seen in adult patients with conventional GBM. Three of the patients in the study with leptomeningeal spread were eventually switched from temozolamide to cisplatin, with moderate responses in each.3

Our case of GBM-PNET was treated with 3 successive rounds of combined radiation and temozolamide therapy, including an SRS boost, with salvage PVC and bevacizumab therapy. This approach resulted in modest local responses and control. This patient, whose age, performance status, and therapy would suggest a median overall survival of 16-21 months, survived 28 months from initial resection.9 The patient did experience a prolonged hospitalization for treatment of acute meningitis, but his neurocognitive and functional deficits remained mild and transient until late in his progression. Other than fatigue noted around the late and subsequent weeks of his radiation treatments, which was milder than expected, this patient experienced very minimal neurocognitive decline until his last few weeks of life, and, outside of his infection-related hospitalization, only rarely reported episodes of headache, nausea, or other forms of acute toxicity. Nevertheless, all therapies ultimately failed to control leptomeningeal spread. Although this is insufficient data to provide conclusive therapeutic recommendations, given the differences in metastatic potential between GBM and PNET tumors, it can be speculated that patients with histologically confirmed GBM with PNET features may benefit from craniospinal irradiation, a commonly used therapy for treating PNETs and medulloblastoma, or the early introduction of platinum-based chemotherapy.3,10

Our experience is consistent with the scant literature and clinical guidelines concerning management of GBM-PNETs. Conventional GBM tumors rarely undergo metastatic spread, and standard management is directed toward the control of local disease. As identifying PNET components in GBMs may have prognostic and treatment ramifications, the method of tumor sampling during biopsy may influence management considerations. Tumors are spatially heterogeneous; therefore, determining both the distribution and functionality of PNET foci within primary lesions is likely help predict clinical behavior. One study suggests that the diffusion coefficient on MR imaging may help distinguish astrocytic and ectodermal tumor components, potentially guiding biopsies.


GBM-PNET tumors are aggressive neoplasms of mixed embryologic origin that demonstrate high rates of local recurrence and dissemination into the CSF. Our patient with GBM-PNET was treated with multiple courses of surgery, radiation and chemotherapy. Therapy provided favorable local responses with an overall survival of 28 months after extensive therapy without debilitating morbidity. The patient tolerated his multiple courses of radiation therapy with minimal complaints of acute toxicity, and, unlike many high-grade glioma patients who do not survive long enough to experience late toxicities, he experienced late toxicity only in the last weeks of his survival. The patient did, despite all aggressive therapy, eventually manifest leptomeningeal spread, which led to a rapid symptomatic decline and death from his disease. This case suggests that aggressive therapies may be utilized in attempts to prolong survival with acceptable toxicity during this period in select patients with favorable performance status. And furthermore, patients with diagnoses of GBM-PNET may benefit from advanced techniques of diagnostic imaging and pathologic analysis, as well as craniospinal irradiation and early platinum-based chemotherapy due to the propensity for CSF dissemination.


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Chu A, Bourgeois DJ, Prasad D.  A responsive yet persistently recurrent GBM with PNET features.  Appl Rad Oncol.  2015;4(4):28-30.

About the Author

Allen Chu, PhD; Daniel J. Bourgeois, III, MD, MPH; Dheerendra Prasad, MD, MCh, FACRO

Allen Chu, PhD; Daniel J. Bourgeois, III, MD, MPH; Dheerendra Prasad, MD, MCh, FACRO

Prepared by Dr. Chu, a 4th Year Medical Student at the Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, SUNY, Buffalo, New York; Dr. Bourgeois, a PGY-4 Resident Physician in Radiation Oncology at Roswell Park Cancer Institute, Buffalo, New York; and Dr. Prasad, Professor of Radiation Medicine, Neurosurgery, and Oncology; Staff Physician in the Radiation Oncology Department; Pediatric Radiation Director; Gamma Knife Center Director; and Medical Director of the Radiation Medicine Department, Roswell Park Cancer Institute.


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